Iranian Company Produces Core Enzyme for Laboratory Test Kits
Core enzymes comprise subunits of enzymes required for catalytic activity.
Ali Gholami, the managing director of the company, referred to the detection of medical samples and diagnosis of infectious cases as other uses of the enzyme.
The enzyme is also used in research and academic studies, he added.
It is produced through recombining and constructing the enzyme gene with a promoter and cloning in a plasmid, transforming the plasmid inside an expression bacterium and producing an enzyme-producing bacterium.
Continuously cultivating and growing bacteria, purifying the enzyme by HPLC and isolated protein, and applying the HotStart feature are also some production stages of the enzyme.
HotStart polymerase is an innovative antibody-modified thermostable enzyme.
The activity of the enzyme is completely closed at room temperature, avoiding the non-specific amplification and primer dimer in the preparation of the sample and the first cycle reaction temperature rise, increasing the specificity of DNA amplification.
The HotStart enzyme is currently imported from the United States, Germany, China, England, and Canada, Gholami pointed out.
4155/g
